Plasmid
Part:BBa_K5302014:Design
Designed by: Bojun Yang Group: iGEM24_USTC (2024-10-01)
pBBR-intimin-mCherry
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4483
Illegal EcoRI site found at 4531
Illegal EcoRI site found at 4804
Illegal PstI site found at 2016
Illegal PstI site found at 3494
Illegal PstI site found at 3742
Illegal PstI site found at 4189
Illegal PstI site found at 4792 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4483
Illegal EcoRI site found at 4531
Illegal EcoRI site found at 4804
Illegal PstI site found at 2016
Illegal PstI site found at 3494
Illegal PstI site found at 3742
Illegal PstI site found at 4189
Illegal PstI site found at 4792
Illegal NotI site found at 1057 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4483
Illegal EcoRI site found at 4531
Illegal EcoRI site found at 4804
Illegal BglII site found at 1803
Illegal XhoI site found at 5149 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4483
Illegal EcoRI site found at 4531
Illegal EcoRI site found at 4804
Illegal PstI site found at 2016
Illegal PstI site found at 3494
Illegal PstI site found at 3742
Illegal PstI site found at 4189
Illegal PstI site found at 4792 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4483
Illegal EcoRI site found at 4531
Illegal EcoRI site found at 4804
Illegal PstI site found at 2016
Illegal PstI site found at 3494
Illegal PstI site found at 3742
Illegal PstI site found at 4189
Illegal PstI site found at 4792
Illegal NgoMIV site found at 2467
Illegal NgoMIV site found at 2750
Illegal NgoMIV site found at 5740
Illegal AgeI site found at 5010
Illegal AgeI site found at 5580 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1379
Illegal SapI.rc site found at 2316
Illegal SapI.rc site found at 2526
Design Notes
Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.
Source
intimin: Escherichia coli mCherry: Mushroom coral